Journal: Heliyon

Article Title: Anti Photoaging Mechanism of a Novel Recombinant Human Fibronectin Peptide (rhFNP) Derived from the Extracellular Matrix

doi: 10.1016/j.heliyon.2025.e42730

Figure Lengend Snippet: Fig. 3. rhFNP skin physiological function experiments. A. The inhibitory effect of rhFNP on NHEK photoaging. a1. MMP1, MMP3, and MMP9 mRNA levels in NHEK cells treated with rhFNP for 48 h a2. Col1α1, Col3α1, and LOR mRNA levels in NHEK cells treated with rhFNP for 48 h a3 & a4. Col1α1, Col3α1, and LOR protein levels and semi-quantitative Analysis in NHEK cells treated with rhFNP for 72 h. B. Col1α1 and Eln1 mRNA levels in zebrafish treated with rhFNP for 24 h n = 3, mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001 vs. model.

Article Snippet: Compound 48/80 (C48/80), Lipopolysaccharide (LPS), Dexamethasone (DXMS), 0.33 % neutral red dye (Sigma-Aldrich, St. Louis, MO, USA), sodium tryptophan (Macklin, Shanghai, CN), mouse β-aminohexosidase A (β-Hex A) ELISA Kit (Cusabio, Wuhan, CN), DMEM/DMEM-F12/MEM medium, FBS purch(Gibco, Grand Island, NY, USA), OTC embedding agent (Servicebio, Wuhan, CN), MS Mouse TNF-α ELISA Kit (Elabscience Wuhan, CN), COL1α1 (E8F4L) XP® Rabbit mAb, COL3α1 (E3K9U) Rabbit mAb (CST, Danvers, MA, USA), His-Tag Antibody (Affinity, jiangsu, CN), Loricrin Polyclonal antibody (Proteintech, Wuhan, CN), α-tubulin Recombinant Rabbit mAb (Starter, Hangzhou, CN), phalloidin (Solarbio, beijing, CN), DAPI (Beyotime, Shanghai, CN), Human Fibronectin (FN, Full - length fibronectin extracted from plasma) (Yeasen, Shanghai, CN).

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